Products
ATR antibody
- Product Name
- ATR antibody
- Catalogue No.
- FNab00736
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ataxia telangiectasia and Rad3 related
- Alternative Names
- ATR antibody, FRAP related protein 1 antibody, FRP1 antibody, MEC1 antibody, SCKL antibody, SCKL1 antibody
- UniProt ID
- Q13535
- Observed MW
- 250-290kd
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:300-1:1000; IP: 1:200-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00736(ATR antibody) at dilution of 1:500
- Background
- Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation(IR), ultraviolet light(UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
