Products
ARHGEF5 antibody
- Product Name
- ARHGEF5 antibody
- Catalogue No.
- FNab00563
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Rho guanine nucleotide exchange factor(GEF) 5
- Alternative Names
- TIM antibody
- UniProt ID
- Q12774
- Observed MW
- 60 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab00563(ARHGEF5 antibody) at dilution of 1:500
IP Result of anti-ARHGEF5 (IP:FNab00563, 4ug; Detection:FNab00563 1:500) with mouse kidney tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using FNab00563(ARHGEF5 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- Guanine nucleotide exchange factor which activates Rho GTPases(PubMed:15601624). Strongly activates RHOA(PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1(By similarity). Involved in regulation of cell shape and actin cytoskeletal organization(PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia(PubMed:14662653). Required for SRC-induced podosome formation(By similarity). Involved in positive regulation of immature dendritic cell migration(By similarity).
