Products
ARHGEF1 antibody
- Product Name
- ARHGEF1 antibody
- Catalogue No.
- FNab00558
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Rho guanine nucleotide exchange factor(GEF) 1
- Alternative Names
- ARHGEF1 antibody, GEF1 antibody, LBCL2 antibody, LSC antibody, P115 RHOGEF antibody, p115RhoGEF antibody, SUB1.5 antibody
- UniProt ID
- Q92888
- Observed MW
- 102-110 kDa
Application
- Tested Applications
- ELISA, IHC, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00558(ARHGEF1 antibody) at dilution of 1:1000
IP Result of anti-ARHGEF1 (IP:FNab00558, 4ug; Detection:FNab00558 1:1000) with HEK-293 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human colon using FNab00558(ARHGEF1 antibody) at dilution of 1:50
- Background
- Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12(GNA12) and alpha-13(GNA13) subunits. Acts as GTPase-activating protein(GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor(GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
