Products
API5 antibody
- Product Name
- API5 antibody
- Catalogue No.
- FNab00482
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- apoptosis inhibitor 5
- Alternative Names
- AAC 11 antibody, AAC11 antibody, Antiapoptosis clone 11 protein antibody, API 5 antibody, API5 antibody, apoptosis inhibitor 5 antibody, FIF antibody, Protein XAGL antibody
- UniProt ID
- Q9BZZ5
- Observed MW
- 50-55 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:50 - 1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00482(API5 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using FNab00482(API5 Antibody) at dilution of 1:50
Immunofluorescent analysis of 10% Formaldehyde fixed HepG2 cells using FNab00482 (API5 antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
