Products
APAF1 antibody
- Product Name
- APAF1 antibody
- Catalogue No.
- FNab00470
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- apoptotic peptidase activating factor 1
- Alternative Names
- APAF 1 antibody, APAF1 antibody, CED4 antibody, KIAA0413 antibody
- UniProt ID
- O14727
- Observed MW
- 140 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00470(APAF1 antibody) at dilution of 1:1000
IP Result of anti-APAF1 (IP:FNab00470, 4ug; Detection:FNab00470 1:500) with HEK-293 cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using FNab00470(APAF1 Antibody) at dilution of 1:200
Immunofluorescent analysis of A549 cells using FNab00470 (APAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9(Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
