Products
Amphiregulin antibody
- Product Name
- Amphiregulin antibody
- Catalogue No.
- FNab00374
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- amphiregulin
- Alternative Names
- Amphiregulin antibody, AR antibody, AREG antibody, AREGB antibody, CRDGF antibody, SDGF antibody
- UniProt ID
- P15514
- Observed MW
- 43 kDa
Application
- Tested Applications
- ELISA, IF, WB, IHC,IP
- Recommended dilution
- WB: 1:200-1:2000; IHC: 1:50-1:500; IP: 1:1000-1:10000; IF: 1:10-1:100
Validated Images
MCF-7 cells were subjected to SDS PAGE followed by western blot with FNab00374(AREG Antibody) at dilution of 1:600
IP Result of anti-Amphiregulin (IP:FNab00374, 4ug; Detection:FNab00374 1:2000) with HepG2 cells lysate 3600ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab00374(AREG Antibody) at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- Amphiregulin(AREG) is one of the ligands of the epidermal growth factor receptor(EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms(PMID: 9642297).
