Products
ALKBH5 antibody
- Product Name
- ALKBH5 antibody
- Catalogue No.
- FNab00314
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- alkB, alkylation repair homolog 5(E. coli)
- Alternative Names
- ABH5 antibody, ALKBH5 antibody, FLJ20308 antibody, OFOXD antibody, OFOXD1 antibody
- UniProt ID
- Q6P6C2
- Observed MW
- 51 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:200-1:2000; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00314(ALKBH5 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human lung cancer using FNab00314(ALKBH5 antibody) at dilution of 1:100
- Background
- Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine(m6A) RNA, the most prevalent internal modification of messenger RNA(mRNA) in higher eukaryotes(PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA(in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export(PubMed:23177736). Required for spermatogenesis(By similarity).
