Products
ALKBH1 antibody
- Product Name
- ALKBH1 antibody
- Catalogue No.
- FNab00311
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- alkB, alkylation repair homolog 1(E. coli)
- Alternative Names
- ABH antibody, ABH1 antibody, alkB antibody, ALKBH antibody, ALKBH1 antibody, DNA lyase ABH1 antibody, hABH antibody
- UniProt ID
- Q13686
- Observed MW
- 44 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:200-1:2000; IHC: 1:20-1:200
Validated Images
human placenta tissue were subjected to SDS PAGE followed by western blot with FNab00311(ALKBH1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human colon tissue slide using FNab00311(ALKBH1 Antibody) at dilution of 1:50
- Background
- Dioxygenase that acts as a DNA demethylase(PubMed:18603530). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed:18603530). Specifically demethylates DNA methylated on the 6th position of adenine(N(6)-methyladenosine) DNA. N(6)-methyladenosine(m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing(By similarity). Also able to repair alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation, but with low activity(PubMed:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites(PubMed:19959401). DNA lyase activity does not require alpha-ketoglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product(PubMed:19959401, PubMed:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation(By similarity).
