Products
AKT antibody
- Product Name
- AKT antibody
- Catalogue No.
- FNab00271
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 8H0
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- v-akt murine thymoma viral oncogene homolog 1
- Alternative Names
- AKT antibody, AKT1 antibody, AKT2 antibody, AKT3 antibody, PKB antibody, PKB ALPHA antibody, PRKBA antibody, Protein kinase B antibody, Proto oncogene c Akt antibody, RAC antibody, RAC ALPHA antibody, RAC PK alpha antibody
- UniProt ID
- P31749
- Observed MW
- 56-62 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:1000-1:10000; IP: 1:500-1:5000; IHC: 1:100-1:400; IF: 1:10-1:100
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00271(AKT Antibody) at dilution of 1:2000
IP Result of anti-AKT1 (IP:FNab00271, 3ug; Detection:FNab00271 1:500) with HeLa cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab00271(AKT antibody) at dilution of 1:200
- Background
- The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho-modification.
