Products
AGS3 antibody
- Product Name
- AGS3 antibody
- Catalogue No.
- FNab00221
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- G-protein signaling modulator 1(AGS3-like, C. elegans)
- Alternative Names
- AGS3 antibody, DKFZp727I051 antibody, GPSM1 antibody
- UniProt ID
- Q86YR5
- Observed MW
- 72 kDa
Application
- Tested Applications
- ELISA, IHC, IF, WB
- Recommended dilution
- WB: 1:200-1:2000; IF: 1:10-1:100; IHC: 1:20-1:200
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab00221(AGS3 antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human lung cancer using FNab00221(AGS3 antibody) at dilution of 1:50
- Background
- GPSM1, also named as AGS3, is a receptor-independent G protein activator that has been implicated in multiple biological events such as brain development, neuroplasticity and addiction, cardiac function, Golgi structure/function, macroautophagy and metabolism. It contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory(GPR) motifs in its C-terminal half. It has been shown that AGS3 could regulate the orientation of the mitotic spindle, cAMP production, membrane protein transport, and asymmetric cell division by binding preferentially to inactive Gai/o subunits complexed with guanine dinucleotide phosphate(GDP) at multiple G-protein regulatory or GoLoco motif repeats. It also plays an important anti-apoptotic role through enhancing the phosphorylation of cyclic AMP response element-binding protein(p-CREB).
