Products
AFF4 antibody
- Product Name
- AFF4 antibody
- Catalogue No.
- FNab00197
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- AF4/FMR2 family, member 4
- Alternative Names
- AF4/FMR2 family member 4 antibody, AF4/FMR2 family antibody, member 4 antibody, AF5Q31 antibody, AFF4 antibody, MCEF antibody, Protein AF 5q31 antibody
- UniProt ID
- Q9UHB7
- Observed MW
- 150 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:1000
Validated Images
L02 cells were subjected to SDS PAGE followed by western blot with FNab00197(AFF4 antibody) at dilution of 1:500
IP Result of anti-AFF4 (IP:FNab00197, 4ug; Detection:FNab00197 1:300) with HepG2 cells lysate 3200ug.
- Background
- Key component of the super elongation complex(SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins(ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
