ADHFE1 antibody

Synonyms:ADH8 antibody, ADHFE1 antibody, FLJ32430 antibody, HMFT2263 antibody, HOT antibody
Catalogue No.:FNab00168Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
ADHFE1 antibody
Catalogue No.
FNab00168
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
alcohol dehydrogenase, iron containing, 1
Alternative Names
ADH8 antibody, ADHFE1 antibody, FLJ32430 antibody, HMFT2263 antibody, HOT antibody
UniProt ID
Q8IWW8
Observed MW
50 kDa
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:1000-1:10000; IHC: 1:20-1:200; IP: 1:200-1:1000
Validated Images
mouse heart tissue were subjected to SDS PAGE followed by western blot with FNab00168(ADHFE1 antibody) at dilution of 1:1000
IP Result of anti-ADHFE1 (IP:FNab00168, 4ug; Detection:FNab00168 1:300) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using FNab00168(ADHFE1 Antibody) at dilution of 1:50
Background
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate(GHB) to succinic semialdehyde(SSA) coupled to reduction of 2-ketoglutarate(2-KG) to D-2-hydroxyglutarate(D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate(L-3-OHB) are also substrates for HOT with 10-fold lower activities.