Products
ACTN4 antibody
- Product Name
- ACTN4 antibody
- Catalogue No.
- FNab00123
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- actinin, alpha 4
- Alternative Names
- ACTININ 4 antibody, actinin antibody, alpha 4 antibody, ACTN4 antibody, Alpha actinin 4 antibody, DKFZp686K23158 antibody, F actin cross linking protein antibody, FSGS antibody, FSGS1 antibody, Non muscle alpha actinin 4 antibody
- UniProt ID
- O43707
- Observed MW
- 105 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP, FC
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:20-1:200; IP: 1:500-1:5000; IF: 1:10-1:100
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab00123(ACTN4 antibody) at dilution of 1:500
IP Result of anti-ACTN4 (IP:FNab00123, 5ug; Detection:FNab00123 1:1000) with HeLa cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab00123(ACTN4 antibody) at dilution of 1:100
- Background
- F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein(Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation(PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions(By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA(PubMed:22351778).
