Products
Aconitase 2 antibody
- Product Name
- Aconitase 2 antibody
- Catalogue No.
- FNab00088
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- aconitase 2, mitochondrial
- Alternative Names
- Gene symbol ACO2 Synonyms ACO2 antibody, Aconitase antibody, aconitase 2 antibody, aconitase 2 antibody, mitochondrial antibody, ACONM antibody, Citrate hydro lyase antibody
- UniProt ID
- Q99798
- Observed MW
- 85 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:50-1:200
Validated Images
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab00088(ACO2 antibody) at dilution of 1:400
Immunohistochemistry of paraffin-embedded human lung cancer using FNab00088(ACO2 antibody) at dilution of 1:50
Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using FNab00088(ACO2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration(ICRD).
