Products
ACK1 antibody
- Product Name
- ACK1 antibody
- Catalogue No.
- FNab00086
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tyrosine kinase, non-receptor, 2
- Alternative Names
- TNK2 antibody, ACK1 antibody, ACK antibody, FLJ45547 antibody, ACK 1 antibody
- UniProt ID
- Q07912
- Observed MW
- 70 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:200-1:2000; IHC: 1:20-1:200
Validated Images
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with FNab00086(ACK1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human breast cancer using FNab00086(ACK1 antibody) at dilution of 1:50
- Background
- TNK2, also named as ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.
