- Product Name
- Rat ARNT/HIF-1-beta(Hypoxia-inducible factor 1-beta) ELISA Kit
- Catalogue No.
- Detection Method
- Sandwich ELISA, Double Antibody
- ARNT/HIF-1-beta ELISA Kit allows for the in vitro quantitative determination of ARNT/HIF-1-beta concentrations in plasma, tissue homogenates and other biological fluids.
- < 0.094ng/ml
- UniProt ID
- Standard curve
- 4 ℃ for 6 months
Matrices listed below were spiked with certain level of ARNT/HIF-1-beta and the recovery rates were calculated by comparing the measured value to the expected amount of ARNT/HIF-1-beta in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 85-101 94 EDTA plasma(n=5) 90-105 96 heparin plasma(n=5) 87-101 93
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ARNT/HIF-1-beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16 serum(n=5) 90-100% 85-103% 85-103% 87-94% EDTA plasma(n=5) 82-101% 83-101% 85-101% 84-99% heparin plasma(n=5) 81-98% 80-93% 84-99% 85-99%
- Intra-Assay: CV<8%
- For Research Use Only
- Research Area