Products

Rat ARNT/HIF-1-beta(Hypoxia-inducible factor 1-beta) ELISA Kit

ER1336 Catalogue No.
Reactivity
Rat
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Rat ARNT/HIF-1-beta(Hypoxia-inducible factor 1-beta) ELISA Kit
Catalogue No.
ER1336
Detection Method
Sandwich ELISA, Double Antibody
Application
ARNT/HIF-1-beta ELISA Kit allows for the in vitro quantitative determination of ARNT/HIF-1-beta concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
P41739
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of ARNT/HIF-1-beta and the recovery rates were calculated by comparing the measured value to the expected amount of ARNT/HIF-1-beta in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10194
EDTA plasma(n=5)90-10596
heparin plasma(n=5)87-10193
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ARNT/HIF-1-beta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-100%85-103%85-103%87-94%
EDTA plasma(n=5)82-101%83-101%85-101%84-99%
heparin plasma(n=5)81-98%80-93%84-99%85-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only