Products

Rat Atp5b(ATP synthase subunit beta, mitochondrial) ELISA Kit

ER0484 Catalogue No.
Atp5b ELISA Kit
Reactivity:
Rat
Range:
31.25-2000pg/ml
Sensitivity:
18.75pg/ml
Product Name
Rat Atp5b(ATP synthase subunit beta, mitochondrial) ELISA Kit
Catalogue No.
ER0484
Alias
Atp5b ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Atp5b ELISA Kit allows for the in vitro quantitative determination of Atp5b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
P10719
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of Atp5b and the recovery rates were calculated by comparing the measured value to the expected amount of Atp5b in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10393
EDTA plasma(n=5)97-102100
heparin plasma(n=5)87-10595
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Atp5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)85-104%86-104%86-102%
EDTA plasma(n=5)83-100%83-100%85-99%
heparin plasma(n=5)90-99%84-91%86-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only