Products

Mouse FLG(Filaggrin) ELISA Kit

EM1853 Catalogue No.
Filaggrin ELISA Kit
Reactivity
Mouse
Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Product Name
Mouse FLG(Filaggrin) ELISA Kit
Catalogue No.
EM1853
Alias
Filaggrin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FLG ELISA Kit allows for the in vitro quantitative determination of FLG concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Mouse
UniProt ID
P11088
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of FLG and the recovery rates were calculated by comparing the measured value to the expected amount of FLG in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10594
EDTA plasma(n=5)85-10393
heparin plasma(n=5)85-10091
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FLG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-104%96-104%85-103%95-104%
EDTA plasma(n=5)82-99%89-100%88-100%82-97%
heparin plasma(n=5)89-95%93-99%84-97%82-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only