Products

Mouse RasGAP(Ras GTPase Activating Protein) ELISA Kit

EM1330 Catalogue No.
RasGAP|p195|SAR1|HUMORFA01|IQ motif containing GTPase activating protein 1|KIAA0051|ras GTPase-activating-like protein IQGAP1|RasGAP-like with IQ motifs ELISA Kit
Reactivity
Mouse
Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Product Name
Mouse RasGAP(Ras GTPase Activating Protein) ELISA Kit
Catalogue No.
EM1330
Alias
RasGAP|p195|SAR1|HUMORFA01|IQ motif containing GTPase activating protein 1|KIAA0051|ras GTPase-activating-like protein IQGAP1|RasGAP-like with IQ motifs ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
RasGAP ELISA Kit allows for the in vitro quantitative determination of RasGAP concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Mouse
UniProt ID
Q9Z268
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of RasGAP and the recovery rates were calculated by comparing the measured value to the expected amount of RasGAP in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10495
EDTA plasma(n=5)89-9894
heparin plasma(n=5)94-10599
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RasGAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-93%91-104%85-104%85-101%
EDTA plasma(n=5)84-98%94-98%85-99%88-96%
heparin plasma(n=5)80-99%87-99%81-91%82-91%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only