Mouse ACA-IgM(anti-Cardiolipin Antibody IgM) ELISA Kit

Category: Research Area:
Catalogue No.: 
EM0283
Alias: 
ACA-IgM ELISA Kit, anti-Cardiolipin Antibody IgM ELISA Kit
Species: 
Mouse
Range: 
3.125-200ng/ml
Sensitivity: 
1.875ng/ml
Manual
  • SPECIFICATIONS
Product Name
Mouse ACA-IgM(anti-Cardiolipin Antibody IgM) ELISA Kit
Alias
ACA-IgM ELISA Kit, anti-Cardiolipin Antibody IgM ELISA Kit
Catalogue No.
EM0283
Size
48T/96T
Species
Mouse
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Sandwich ELISA, Double Antigen
Detection Wavelength
OD450
Reaction Duration
3 hours
Range
3.125-200ng/ml
Sensitivity
1.875ng/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with ACA-IgM , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml) OD-1 OD-2 Average
0 0.139 0.143 0.141
3.125 0.207 0.213 0.21
6.25 0.35 0.36 0.355
12.5 0.45 0.464 0.457
25 0.794 0.818 0.806
50 1.279 1.317 1.298
100 1.796 1.848 1.822
200 2.16 2.222 2.191
EM0283 Standard Curve Image
Recovery

Add a certain amount of ACA-IgM into the sample. Calculate the recovery by comparing the measured value with the expected amount of ACA-IgM in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 85-102 94
EDTA plasma(n=10) 89-95 91
Heparin plasma(n=10) 89-97 93
Linearity

Dilute the sample with a certain amount of ACA-IgM at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 91-100% 89-101% 80-97%
EDTA plasma(n=10) 89-102% 84-101% 81-99%
Heparin plasma(n=10) 86-96% 87-99% 80-93%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/ml) 6.25 24.4 96.85 6.43 24.79 99.68
Standard deviation 0.34 1.27 5.64 0.38 1.35 5.84
CV(%) 5.43 5.22 5.82 5.91 5.43 5.86
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100
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