Products
Human HCV IgG(Hepatitis C Virus Antibody IgG) ELISA Kit
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with HCV-Ab bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HCV-Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Product Name
- Human HCV IgG(Hepatitis C Virus Antibody IgG) ELISA Kit
- Alias
- Anti HCV ELISA Kit, Hepatitis C Virus Antibody ELISA Kit
- Catalogue No.
- EH3774
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- HRP indrect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 2 hours
- Range
- Qualitative
- Sensitivity
- Qualitative
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with HCV-Ab , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
