Products

Human C4(Complement Component 4) ELISA Kit

EH2740 Catalogue No.
C4 ELISA Kit
Reactivity
Human
Range
4.688-300ng/ml
Sensitivity
2.813ng/ml
Product Name
Human C4(Complement Component 4) ELISA Kit
Catalogue No.
EH2740
Alias
C4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C4 ELISA Kit allows for the in vitro quantitative determination of C4 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
4.688-300ng/ml
Sensitivity
< 2.813ng/ml
Species
Human
UniProt ID
P0C0L4
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of C4 and the recovery rates were calculated by comparing the measured value to the expected amount of C4 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)92-10499
EDTA plasma(n=5)85-9992
heparin plasma(n=5)85-10592
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-101%90-102%86-104%85-105%
EDTA plasma(n=5)87-101%86-91%84-101%82-99%
heparin plasma(n=5)86-99%83-100%86-99%82-90%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only