Products

Human C3b(Complement Fragment 3b) ELISA Kit

EH2735 Catalogue No.
C3b ELISA Kit
Reactivity:
Human
Range:
0.313-20ng/ml
Sensitivity:
0.188ng/ml
Product Name
Human C3b(Complement Fragment 3b) ELISA Kit
Catalogue No.
EH2735
Alias
C3b ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C3b ELISA Kit allows for the in vitro quantitative determination of C3b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of C3b and the recovery rates were calculated by comparing the measured value to the expected amount of C3b in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)90-9592
EDTA plasma(n=5)86-9894
heparin plasma(n=5)87-10598
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-103%87-105%88-105%
EDTA plasma(n=5)89-94%89-98%84-94%
heparin plasma(n=5)82-96%84-100%80-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only