Products

Human AP(Allopregnanolone) ELISA Kit

EH2648 Catalogue No.
AP ELISA Kit, 3α ELISA Kit, 5α-tetrahydroprogesterone ELISA Kit, 3α-hydroxy-5α-pregnan-20-one ELISA Kit
Reactivity
Human
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Human AP(Allopregnanolone) ELISA Kit
Catalogue No.
EH2648
Alias
AP ELISA Kit, 3α ELISA Kit, 5α-tetrahydroprogesterone ELISA Kit, 3α-hydroxy-5α-pregnan-20-one ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
AP ELISA Kit allows for the in vitro quantitative determination of AP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of AP and the recovery rates were calculated by comparing the measured value to the expected amount of AP in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10295
EDTA plasma(n=5)88-10596
heparin plasma(n=5)85-10093
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)85-105%85-98%86-95%
EDTA plasma(n=5)84-96%89-96%90-100%
heparin plasma(n=5)81-95%83-92%80-92%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only