Products

Human AFM(Afamin) ELISA Kit

EH2493 Catalogue No.
AFM ELISA Kit, ALB2 ALBAALF afamin ALB2alpha-Alb ELISA Kit, ALBAalpha-albumin ELISA Kit, ALF ELISA Kit, Alpha-Alb ELISA Kit, Alpha-albumin ELISA Kit
Reactivity
Human
Range
3.125-200ng/ml
Sensitivity
1.875ng/ml
Product Name
Human AFM(Afamin) ELISA Kit
Catalogue No.
EH2493
Alias
AFM ELISA Kit, ALB2 ALBAALF afamin ALB2alpha-Alb ELISA Kit, ALBAalpha-albumin ELISA Kit, ALF ELISA Kit, Alpha-Alb ELISA Kit, Alpha-albumin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AFM ELISA Kit allows for the in vitro quantitative determination of AFM concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
UniProt ID
P43652
Standard curve
Storage
4 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of AFM and the recovery rates were calculated by comparing the measured value to the expected amount of AFM in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-9692
EDTA plasma(n=5)87-9993
heparin plasma(n=5)85-9991
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AFM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-105%85-103%91-102%85-104%
EDTA plasma(n=5)82-89%83-93%85-90%82-101%
heparin plasma(n=5)81-98%80-86%88-99%84-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only