Products

Human 4-HNE(4-Hydroxynonenal) ELISA Kit

EH2080 Catalogue No.
4-HNE(4-Hydroxynonenal)|HNE|4 HNE ELISA Kit
Reactivity
Human
Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Product Name
Human 4-HNE(4-Hydroxynonenal) ELISA Kit
Catalogue No.
EH2080
Alias
4-HNE(4-Hydroxynonenal)|HNE|4 HNE ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
4-HNE ELISA Kit allows for the in vitro quantitative determination of 4-HNE concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)92-10498
EDTA plasma(n=5)89-10397
heparin plasma(n=5)86-10393
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-105%86-98%89-102%86-101%
EDTA plasma(n=5)82-101%83-96%85-99%87-101%
heparin plasma(n=5)84-96%83-96%87-100%80-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only