Products

Human GAPDH(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit

EH2006 Catalogue No.
GAPDH|Glyceraldehyde-3-phosphate dehydrogenase|Peptidyl-cysteine S-nitrosylase GAPDH|GAPD|G3PD|G3PDH|aging-associated gene 9 protein ELISA Kit
Reactivity
Human
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Human GAPDH(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit
Catalogue No.
EH2006
Alias
GAPDH|Glyceraldehyde-3-phosphate dehydrogenase|Peptidyl-cysteine S-nitrosylase GAPDH|GAPD|G3PD|G3PDH|aging-associated gene 9 protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
GAPDH ELISA Kit allows for the in vitro quantitative determination of GAPDH concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
P04406
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of GAPDH and the recovery rates were calculated by comparing the measured value to the expected amount of GAPDH in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)91-9894
EDTA plasma(n=5)89-10493
heparin plasma(n=5)85-9993
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAPDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)94-103%85-104%94-102%99-103%
EDTA plasma(n=5)83-95%85-100%82-99%82-97%
heparin plasma(n=5)83-98%84-98%82-99%84-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only