Products

Human 12-LO(Arachidonate 12-Lipoxygenase) ELISA Kit

EH1980 Catalogue No.
12-LO ELISA Kit, Arachidonate 12-Lipoxygenase ELISA Kit, ALOX12 ELISA Kit, LOG12 ELISA Kit, 12S-LOX ELISA Kit, 12S-lipoxygenase ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit
Reactivity
Human
Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Product Name
Human 12-LO(Arachidonate 12-Lipoxygenase) ELISA Kit
Catalogue No.
EH1980
Alias
12-LO ELISA Kit, Arachidonate 12-Lipoxygenase ELISA Kit, ALOX12 ELISA Kit, LOG12 ELISA Kit, 12S-LOX ELISA Kit, 12S-lipoxygenase ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
12-LO ELISA Kit allows for the in vitro quantitative determination of 12-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
P18054
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of 12-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 12-LO in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)94-104100
EDTA plasma(n=5)88-10398
heparin plasma(n=5)85-10393
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 12-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)87-101%85-105%87-103%
EDTA plasma(n=5)82-99%87-101%83-93%
heparin plasma(n=5)80-99%83-98%84-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only