Products

Human LIG3(DNA ligase 3) ELISA Kit

EH1715 Catalogue No.
LIG3|DNA ligase 3|DNA ligase III|Polydeoxyribonucleotide synthase [ATP] 3 ELISA Kit
Reactivity
Human
Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Product Name
Human LIG3(DNA ligase 3) ELISA Kit
Catalogue No.
EH1715
Alias
LIG3|DNA ligase 3|DNA ligase III|Polydeoxyribonucleotide synthase [ATP] 3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
LIG3 ELISA Kit allows for the in vitro quantitative determination of LIG3 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
P49916
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of LIG3 and the recovery rates were calculated by comparing the measured value to the expected amount of LIG3 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10293
EDTA plasma(n=5)86-10396
heparin plasma(n=5)89-10598
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LIG3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)92-103%85-92%88-105%85-104%
EDTA plasma(n=5)82-101%90-97%83-99%83-97%
heparin plasma(n=5)84-100%83-99%84-95%83-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only