Products

Human FLG(Filaggrin) ELISA Kit

EH1353 Catalogue No.
FLG|Filaggrin|ATOD2 ELISA Kit
Reactivity
Human
Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Product Name
Human FLG(Filaggrin) ELISA Kit
Catalogue No.
EH1353
Alias
FLG|Filaggrin|ATOD2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FLG ELISA Kit allows for the in vitro quantitative determination of FLG concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
P20930
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of FLG and the recovery rates were calculated by comparing the measured value to the expected amount of FLG in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)97-104100
EDTA plasma(n=5)86-10596
heparin plasma(n=5)91-10097
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FLG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-103%86-103%85-96%85-100%
EDTA plasma(n=5)84-97%86-99%86-100%82-97%
heparin plasma(n=5)86-93%80-93%81-96%82-100%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only