Human ACTN3(Alpha-actinin-3) ELISA Kit
EH1251
Catalogue No.
- ACTN3 ELISA Kit, Actinin Alpha 3 ELISA Kit, alpha-actinin skeletal muscle isoform 3 ELISA Kit, F-actin cross-linking protein ELISA Kit
- Reactivity
- Human
- Range
- 0.313-20ng/ml
- Sensitivity
- 0.188ng/ml
- Product Name
- Human ACTN3(Alpha-actinin-3) ELISA Kit
- Catalogue No.
- EH1251
- Alias
- ACTN3 ELISA Kit, Actinin Alpha 3 ELISA Kit, alpha-actinin skeletal muscle isoform 3 ELISA Kit, F-actin cross-linking protein ELISA Kit
- Detection Method
- Sandwich ELISA, Double Antibody
- Application
- ACTN3 ELISA Kit allows for the in vitro quantitative determination of ACTN3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
- Size
- 96T
- Range
- 0.313-20ng/ml
- Sensitivity
- < 0.188ng/ml
- Species
- Human
- UniProt ID
- Q08043
- Standard curve
- Storage
- 2-8 ℃ for 6 months
- Recovery
Matrices listed below were spiked with certain level of ACTN3 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTN3 in samples.
Matrix Recovery range(%) Average(%) serum(n=5) 88-105 99 EDTA plasma(n=5) 88-99 96 heparin plasma(n=5) 87-100 96 - Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 serum(n=5) 90-105% 85-101% 86-100% EDTA plasma(n=5) 87-100% 82-98% 86-99% heparin plasma(n=5) 85-97% 89-99% 82-99% - CV(%)
- Intra-Assay: CV<8%
Inter-Assay: CV<10% - Note
- For Research Use Only