Products

Human ACTN3(Alpha-actinin-3) ELISA Kit

EH1251 Catalogue No.
ACTN3 ELISA Kit, Actinin Alpha 3 ELISA Kit, alpha-actinin skeletal muscle isoform 3 ELISA Kit, F-actin cross-linking protein ELISA Kit
Reactivity
Human
Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Product Name
Human ACTN3(Alpha-actinin-3) ELISA Kit
Catalogue No.
EH1251
Alias
ACTN3 ELISA Kit, Actinin Alpha 3 ELISA Kit, alpha-actinin skeletal muscle isoform 3 ELISA Kit, F-actin cross-linking protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ACTN3 ELISA Kit allows for the in vitro quantitative determination of ACTN3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Q08043
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of ACTN3 and the recovery rates were calculated by comparing the measured value to the expected amount of ACTN3 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-10599
EDTA plasma(n=5)88-9996
heparin plasma(n=5)87-10096
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTN3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)90-105%85-101%86-100%
EDTA plasma(n=5)87-100%82-98%86-99%
heparin plasma(n=5)85-97%89-99%82-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only