Products

Human F8(Coagulation factor VIII) ELISA Kit

EH1068 Catalogue No.
F8 ELISA Kit, Coagulation Factor Ⅷ ELISA Kit, F8C ELISA Kit, Antihemophilic factor ELISA Kit, AHF ELISA Kit, Procoagulant component ELISA Kit
Reactivity
Human
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Human F8(Coagulation factor VIII) ELISA Kit
Catalogue No.
EH1068
Alias
F8 ELISA Kit, Coagulation Factor Ⅷ ELISA Kit, F8C ELISA Kit, Antihemophilic factor ELISA Kit, AHF ELISA Kit, Procoagulant component ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
F8 ELISA Kit allows for the in vitro quantitative determination of F8 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
P00451
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of F8 and the recovery rates were calculated by comparing the measured value to the expected amount of F8 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)86-10295
EDTA plasma(n=5)88-10597
heparin plasma(n=5)85-10291
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)88-91%86-95%85-99%
EDTA plasma(n=5)84-99%84-101%87-100%
heparin plasma(n=5)82-93%81-99%84-93%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only