Products

Human 15-LO(Arachidonate 15-Lipoxygenase) ELISA Kit

EH0204 Catalogue No.
15-LO ELISA Kit, ALOX15 ELISA Kit, 15-LOX-1 ELISA Kit, 15LOX-1 ELISA Kit, LOX15 ELISA Kit
Reactivity:
Human
Range:
62.5-4000pg/ml
Sensitivity:
37.5pg/ml
Product Name
Human 15-LO(Arachidonate 15-Lipoxygenase) ELISA Kit
Catalogue No.
EH0204
Alias
15-LO ELISA Kit, ALOX15 ELISA Kit, 15-LOX-1 ELISA Kit, 15LOX-1 ELISA Kit, LOX15 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
15-LO ELISA Kit allows for the in vitro quantitative determination of 15-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Human
UniProt ID
P16050
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of 15-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 15-LO in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-9490
EDTA plasma(n=5)88-10595
heparin plasma(n=5)94-10598
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:8
serum(n=5)86-102%85-103%92-98%
EDTA plasma(n=5)85-99%85-94%84-101%
heparin plasma(n=5)82-94%81-86%80-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only