Products

Human CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

EH0086 Catalogue No.
CD40L|TNFSF5|TNFSF5|CD40LG|CD154|HIGM1|IGM|IMD3|T-BAM|TRAP|Gp39 ELISA Kit
Reactivity
Human
Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Product Name
Human CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit
Catalogue No.
EH0086
Alias
CD40L|TNFSF5|TNFSF5|CD40LG|CD154|HIGM1|IGM|IMD3|T-BAM|TRAP|Gp39 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Human
UniProt ID
P29965
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of CD40L and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)85-10293
EDTA plasma(n=5)94-10399
heparin plasma(n=5)85-10594
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-102%94-102%85-97%94-105%
EDTA plasma(n=5)89-99%89-101%82-99%86-96%
heparin plasma(n=5)81-97%85-98%84-99%80-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only