Products

Chicken CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

ECH0014 Catalogue No.
CD40L|TNFSF5|TNFSF5|CD40LG|CD154|HIGM1|IGM|IMD3|T-BAM|TRAP|Gp39 ELISA Kit
Reactivity
Chicken
Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Product Name
Chicken CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit
Catalogue No.
ECH0014
Alias
CD40L|TNFSF5|TNFSF5|CD40LG|CD154|HIGM1|IGM|IMD3|T-BAM|TRAP|Gp39 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L/TNFSF5 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Chicken
UniProt ID
Q9I8D8
Standard curve
Storage
2-8 ℃ for 6 months
Recovery

Matrices listed below were spiked with certain level of CD40L/TNFSF5 and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L/TNFSF5 in samples.

MatrixRecovery range(%)Average(%)
serum(n=5)88-9592
EDTA plasma(n=5)85-10590
heparin plasma(n=5)89-10397
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L/TNFSF5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)86-93%87-105%89-105%85-105%
EDTA plasma(n=5)86-96%87-96%82-92%82-97%
heparin plasma(n=5)82-92%83-90%88-100%88-96%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only