Mouse Anti-MPV IgM(Anti-Monkeypox Virus IgM) ELISA Kit

This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Anti-MPV IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-MPV IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Catalogue No.: 
MPXV IgM ELISA Kit, Monkeypox virus IgM ELISA Kit
Product Name
Mouse Anti-MPV IgM(Anti-Monkeypox Virus IgM) ELISA Kit
MPXV IgM ELISA Kit, Monkeypox virus IgM ELISA Kit
Catalogue No.
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Indirect ELISA
Detection Wavelength
Reaction Duration
1.5 hours
2-8°C(Sealed), Don't cryopreserve.
Specifically binds with Anti-MPV IgM , no obvious cross reaction with other analogues.
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Assay Procedure Summary
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml) OD-1 OD-2 Average
0 0.103 0.107 0.105
1.562 0.17 0.174 0.172
3.125 0.227 0.233 0.23
6.25 0.37 0.38 0.375
12.5 0.587 0.605 0.596
25 0.971 0.999 0.985
50 1.694 1.744 1.719
100 2.232 2.296 2.264
EM2128 Standard Curve Image

Add a certain amount of Anti-MPV IgM into the sample. Calculate the recovery by comparing the measured value with the expected amount of Anti-MPV IgM in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 96-102 100
EDTA plasma(n=10) 86-103 97
Heparin plasma(n=10) 85-102 96

Dilute the sample with a certain amount of Anti-MPV IgM at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 88-102% 85-92% 91-100%
EDTA plasma(n=10) 85-104% 89-100% 85-97%
Heparin plasma(n=10) 86-95% 87-100% 88-99%

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/ml) 3.07 12.18 47.85 3.11 11.87 46.35
Standard deviation 0.14 0.63 2.19 0.17 0.7 1.97
CV(%) 4.58 5.16 4.58 5.32 5.86 4.26

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100