[Cited FineTest ELISA Kit] Current Research on Drug-induced Colitis Treatment

FineTest ELISA kit contributes to the research on drug-induced colitis treatment. The immunoassay is designed to measure TNF-α, IL-1β, IL-10, and Arg-1 concentration in culture supernatant.

Publication Details
Article Title: Hyaluronic acid modified oral drug delivery system with mucoadhesiveness and macrophage-targeting for colitis treatment
Journal Title: Carbohydrate Polymers
DOI: 10.1016/j.carbpol.2023.120884
IF: 10.723

Abstract:   Based on the biocompatibility and macrophage targeting of natural polysaccharides, combined with the physiological and pathological characteristics of the gastrointestinal tract and colonic mucosa of ulcerative colitis (UC), we prepare dexamethasone (Dex)-loaded oral colon-targeted nano-in-micro drug delivery systems coated with multilayers of chitosan (CS), hyaluronic acid (HA), and finally Eudragit S100 (ECHCD MPs) using a layer-by-layer coating technique for UC treatment through regulating the M1/M2 polarization of intestinal macrophages. HA/CS/Dex nanoparticles (HCD NPs) are ingested by macrophages via CD44 receptor-mediated endocytosis to regulate M1-to-M2 macrophage polarization and exert anti-inflammatory effects. Moreover, ECHCD MPs show better colon-targeting properties than Dex-loaded chitosan nanoparticles (CD NPs) and HCD NPs which is demonstrated by stronger mucoadhesion to inflamed colon tissues. After oral administration, ECHCD MPs exert significant anti-UC effects. Therefore, ECHCD MPs are proven to be as promising oral colon-targeting drug delivery systems for Dex and have potential application in UC treatment.

Keywords:    Colitis treatment, Dexamethasone, Natural polysaccharides, Nano-in-micro delivery system, Colon targeting, Macrophage polarization

Immunoassay

Illustration

Figure Source: Carbohydr Polym. 2023. doi: 10.1016/j.carbpol.2023.120884.