FineTest ELISA kit contributes to the research on cancer immunotherapy. The immunoassay is designed to measure HMGB1 concentration in supernatant.
Publication Details
Article Title: Microbial synthesis of Prussian blue for potentiating checkpoint blockade immunotherapy
Journal Title: Nature Communications
DOI: 10.1038/s41467-023-38796-9
IF: 17.69
PMID: 37221237
Abstract: Cancer immunotherapy is revolutionizing oncology. The marriage of nanotechnology and immunotherapy offers a great opportunity to amplify antitumor immune response in a safe and effective manner. Here, electrochemically active Shewanella oneidensis MR-1 can be applied to produce FDA-approved Prussian blue nanoparticles on a large-scale. We present a mitochondria-targeting nanoplatform, MiBaMc, which consists of Prussian blue decorated bacteria membrane fragments having further modifications with chlorin e6 and triphenylphosphine. We find that MiBaMc specifically targets mitochondria and induces amplified photo-damages and immunogenic cell death of tumor cells under light irradiation. The released tumor antigens subsequently promote the maturation of dendritic cells in tumor-draining lymph nodes, eliciting T cell-mediated immune response. In two tumor-bearing mouse models using female mice, MiBaMc triggered phototherapy synergizes with anti-PDL1 blocking antibody for enhanced tumor inhibition. Collectively, the present study demonstrates biological precipitation synthetic strategy of targeted nanoparticles holds great potential for the preparation of microbial membrane-based nanoplatforms to boost antitumor immunity.
Keywords: Biomedical materials, Biosynthesis, Cancer immunotherapy, Metal–organic frameworks, Tumour immunology
Immunoassay
| FineTest Product | Sample | Detection Target |
| Mouse Hmgb1(High mobility group protein B1) ELISA Kit(EM0382) | supernatant | HMGB1 |
Validated Image
Figure Source: Nat Commun. 2023 May 23;14(1):2943. doi: 10.1038/s41467-023-38796-9.
Fig. 3: HMGB1 in culture medium after different treatments (n = 3 independent experiments). Statistical analysis was conducted with ANOVA with Tukey’s tests. *P < 0.05, **P < 0.01. ***P < 0.001, ****P < 0.0001.