[Cited FineTest ELISA Kit] Current Research on Alzheimer’s Disease

FineTest ELISA kit contributes to the research on Alzheimer's disease.The immunoassay is designed to measure IL-1β and IL-18 concentration in hippocampus.

Publication Details
Article Title: MCC950 reduces autophagy and improves cognitive function by inhibiting NLRP3-dependent neuroinflammation in a rat model of Alzheimer's disease
Journal Title: Brain, Behavior, and Immunity
DOI: 10.1016/j.bbi.2023.11.031
IF: 15.1
PMID: 38040385

Abstract: Alzheimer's disease (AD) is the seventh most common cause of mortality and one of the major causes of disability and vulnerability in the elderly. AD is characterized by gradual cognitive deterioration, the buildup of misfolded amyloid beta (Aβ) peptide, and the generation of neurofibrillary tangles. Despite enormous scientific progress, there is no effective cure for AD. Thus, exploring new treatment options to stop AD or at least slow down its progress is important. In this study, we investigated the potential therapeutic effects of MCC950 on NLRP3-mediated inflammasome-driven inflammation and autophagy in AD. Rats treated with streptozotocin (STZ) exhibited simultaneous activation of the NLRP3 inflammasome and autophagy, as confirmed by Western blot, immunofluorescence, and co-immunoprecipitation analyses. MCC950, a specific NLRP3 inhibitor, was intraperitoneally administered (50 mg/kg body weight) to rats with AD-like symptoms induced by intracerebroventricular STZ injections (3 mg/kg body weight). MCC950 effectively suppressed STZ-induced cognitive impairment and anxiety by inhibiting NLRP3-dependent neuroinflammation. Moreover, our findings indicate that MCC950 exerts neuroprotective effects by attenuating autophagy in neuronal cells. The inhibiting effects of MCC950 on inflammasome activation and autophagy were reproduced in vitro, provding further mechansistic insights into MCC950 therapeutic action. Our findings suggest that MCC950 impedes the progression of AD and may also improve cognitive function through the mitigation of autophagy and NLRP3 inflammasome inhibition.

Keywords: Alzheimer's disease, Amyloid-beta, Autophagy, Inflammation, MCC950, NLRP3

Immunoassay

Illustration

Figure Source: Brain Behav Immun. 2023 Nov 29:116:70-84. doi: 10.1016/j.bbi.2023.11.031.