【Cited FineTest Elisa Kit】 Current Research on Immunosuppression Therapy

FineTest Elisa kit contributes to the research on immunosuppression therapy. The immunoassay is designed to measure GAL9 and Hmgb1 concentration in homogenate of thymus, spleen, lung, liver and kidney.

Publication Details
Article Title: Tim-3 regulates sepsis-induced immunosuppression by inhibiting the NF-κB signaling pathway in CD4 T cells
Journal Title: Molecular Therapy
DOI: 10.1016/j.ymthe.2021.12.013
IF: 11.454
PMID: 34933101

Abstract:     Immunosuppression in response to severe sepsis remains a serious human health concern. Evidence of sepsis-induced immunosuppression includes impaired T lymphocyte function, T lymphocyte depletion or exhaustion, increased susceptibility to opportunistic nosocomial infection, and imbalanced cytokine secretion. CD4 T cells play a critical role in cellular and humoral immune responses during sepsis. Here, using an RNA sequencing assay, we found that the expression of T cell-containing immunoglobulin and mucin domain-3 (Tim-3) on CD4 T cells in sepsis-induced immunosuppression patients was significantly elevated. Furthermore, the percentage of Tim-3⁺ CD4 T cells from sepsis patients was correlated with the mortality of sepsis-induced immunosuppression. Conditional deletion of Tim-3 in CD4 T cells and systemic Tim-3 deletion both reduced mortality in response to sepsis in mice by preserving organ function. Tim-3⁺ CD4 T cells exhibited reduced proliferative ability and elevated expression of inhibitory markers compared with Tim-3^-CD4 T cells. Colocalization analyses indicated that HMGB1 was a ligand that binds to Tim-3 on CD4 T cells and that its binding inhibited the NF-κB signaling pathway in Tim-3⁺ CD4 T cells during sepsis-induced immunosuppression. Together, our findings reveal the mechanism of Tim-3 in regulating sepsis-induced immunosuppression and provide a novel therapeutic target for this condition.
Keywords:  CD4 T cells, T cell-Containing Immunoglobulin and Mucin Domain-3 (Tim-3), Biomarker, Sepsis-induced Immunosuppression, Immunosuppression Therapy

Immunoassay