Products
APC Anti-Human HLA-G Antibody(87G)
| Synonyms： | HLA class I histocompatibility antigen|alpha chain G|HLA G antigen|MHC class I antigen G|Soluble HLA class I histocompatibility antigen|alpha chain G (sHLA-G)|HLA-G|HLA-6.0|HLAG antibody | ||
| Catalogue No.： | APC-30434 | Reactivity： | Human |
| Host： | Mouse | Tested Application： | FC |
| Clonality： | monoclonal | Isotype： | IgG2a,κ |
| Size | Price |
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- SPECIFICATIONS
- FIGURES
- CONDITIONS
- FAQS
- Product Name
- APC Anti-Human HLA-G Antibody(87G)
- Catalogue No.
- APC-30434
- Size
- 25T/100T
- Form
- liquid
- Conjugation
- APC
- Conjugation Information
- APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
- Clonality
- monoclonal
- Isotype
- IgG2a,κ
- Clone ID
- 87G
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
- Alternative Names
- HLA class I histocompatibility antigen|alpha chain G|HLA G antigen|MHC class I antigen G|Soluble HLA class I histocompatibility antigen|alpha chain G (sHLA-G)|HLA-G|HLA-6.0|HLAG antibody
- UniProt ID
- P17693
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
- Background
- HLA-G Antibody (4H84) is an IgG1 κ mouse monoclonal HLA-G antibody (also designated HLA major histocompatibility complex,class I,G antibody,human leukocyte antigen (HLA) G antibody,45 kDa transmembrane class I human leukocyte antigen G antibody,chromosome 6p22 gene complex class 1G antibody,placenta trophoblast marker antibody,or fetal derived placental cell marker antibody) that detects the HLA-G protein of mouse,rat and human origin by WB,IP,IF and IHC(P). HLA-G Antibody (4H84) is available as both the non-conjugated anti-HLA-G antibody form,as well as multiple conjugated forms of anti-HLA-G antibody,including agarose,HRP,PE,FITC and multiple Alexa Fluor® conjugates. Major histocompatibility complex (MHC),human leukocyte antigen (HLA) molecules are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains,an α or heavy chain,and a non-covalently associated protein,β-2-microglobulin. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells,HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
How many times can antibodies be recycled?
First, usually it's not suggested to recycle antibodies. After use, buffer system of antibodies has changed. The storage condition of recycled antibodies for different customers also varies. Thus, the performance efficiency of recycled antibodies can’t be guaranteed. Besides, FineTest ever conducted the antibody recycling assay. Assay results show recycling times of different antibodies also varies. Usually, higher antibody titer allows more repeated use. Customers can determine based on experimental requirements.
Notes: After incubation, we recycle rest antibodies to centrifuge tube and store at 4℃. High titer antibodies can be stored for a minimum of one week. Reuse about three times.
What are components of FineTest antibody buffer?
Components of FineTest antibody buffer are usually PBS with proclin300 or sodium azide, BSA, 50% glycerol. Common preservative is proclin300 or sodium azide, which is widely applied in the lab and industry.
How about the storage temperature and duration of FineTest antibodies?
Most antibodies are stored at -20℃. Directly-labeled flow cytometry antibodies should be stored at 2 - 8℃. The shelf life is one year. If after sales issues for purchased antibodies appear, return or replacement is available. Usually, antibodies can be still used after the one-year warranty. We can offer technical support services.
Is dilution required for FineTest antibodies? What’s the dilute solution?
Directly-labeled flow cytometry antibodies are ready-to-use without dilution. Other antibodies are usually concentrated. Follow the dilution ratio suggested in the manual. Dilute solution for different experiments also varies. Common antibody dilution buffers are acceptable(e.g. PBST, TBST, antibody blocking buffer).
How to retrieve antibodies for immunohistochemistry?
Common retrieval buffers: Tris-EDTA Buffer(pH 9.0); Citrate Buffer(pH 6.0)
Heat induced antibody retrieval:
Method 1: Water-bath heating: Put the beaker with retrieval buffer and slide in the boiling water bath. Keep the boiling state for 15min. Naturally cool to room temperature;
Method 2: Microwave retrieval: Put the beaker with retrieval buffer and slide in the microwave oven. Heat at high power for 5min, Switch OFF for 3min, Heat at medium power for 5min. Naturally cool to room temperature.
How to choose secondary antibodies?
(1) Secondary antibodies react with primary antibodies. Thus, secondary antibodies should be against host species of primary antibodies. E.g. If the primary antibody is derived from rabbit, the relevant secondary antibody should be against rabbit. E.g. goat anti rabbit or donkey anti rabbit.
(2) Choose secondary antibody conjugates according to the experimental type, e.g. ELISA, WB, IHC etc. Common enzyme conjugated secondary antibodies are labelled by HRP, AP etc. Fluorescin or dye labelled secondary antibodies are applied in immunofluorescence and flow cytometry(e.g. FITC, Cy3).