Pyruvic Acid Colorimetric Assay Kit

K140

96T(80 samples)

0.003 μmol/mL

0.003-2.0 μmol/mL

This kit is suitable for detecting the content of pyruvic acid in serum (plasma), as well as in animal and plant tissues.

The kit should be stored at 2-8℃ shading light for 6 months.

Note: The reagents should be stored strictly according to the preservation conditions listed in the table above. The reagents in different test kits cannot be mixed. For reagents with a small volume, please centrifuge them before use to ensure that an adequate amount of reagent can be obtained.

Instruments: Microplate reader (480-520 nm, optimum wavelength: 505 nm)

Reagents:Double distilled water, Normal saline (0.9% NaCl)or PBS(0.01 M，pH 7.4)

1.Equilibrate all the reagents to room temperature before use.

2.The preparation of standard curve：

1.Sample preparation:

Serum and plasma samples: Can be directly measured.

Tissue samples: Take 0.020 - 1.0 g of fresh tissue blocks, add 0.9% NaCl or PBS (0.01 M, pH 7.4) to a ratio of weight (g) : volume (mL) = 1:9, homogenize, centrifuge at 4℃, 10000 × g for 10 minutes, take the supernatant and place it on ice for measurement. Some of the supernatant is retained for protein concentration determination.

2.Dilution of sample:

Before the formal test, 2-3 samples with expected significant differences should be diluted to different concentrations for a preliminary experiment. Based on the results of the preliminary experiment and in combination with the linear range of this kit: 0.003 - 2.0 μmol/mL, the dilution can be carried out according to the table below (for reference only)：

Note: The dilution solution is PBS (0.01 M, pH 7.4) or normal saline (0.9% NaCl).

Standard curve for the sample: y = ax + b

Calculation of pyruvic acid in serum (plasma) and other liquid samples:

Pyruvic acid content (μmol/mL) = (ΔA505 - b) ÷ a × f

Calculation of pyruvic acid in tissues:

Pyruvic acid content (μmol/mgprot) = (ΔA505 - b) ÷ a × f ÷ Cpr

Note:

y: OD value of the standard sample - OD value of the blank (OD value of the standard sample when its concentration is 0)

x: Concentration corresponding to the absorbance

a: Slope of the standard curve

b: Intercept of the standard curve

ΔA505: OD value of the test well - OD value of the blank, the OD value of the blank is the OD value when the concentration of the standard sample is 0

f: Dilution factor before adding the sample to the detection system

Cpr: Protein concentration of the sample to be tested (mg/mL)

1. The kit is for research purposes only. If it is used for clinical diagnosis or any other purposes, our company will not be responsible for any problems arising therefrom, nor will it bear any legal liability.

2. Please read the instructions carefully before the experiment and adjust the equipment properly. Conduct the experiment strictly in accordance with the instructions.

3. During the experiment, please wear laboratory coats and latex gloves to ensure protection.

4. The detection range of the kit is not equivalent to the concentration range of the analyte in the sample. If the concentration of the analyte in the sample is too high or too low, please appropriately dilute or concentrate the sample.

5. If the sample to be tested is not among the sample types listed in the instructions, it is recommended to conduct a preliminary experiment to verify the detection validity.

6. The final experimental results are closely related to the effectiveness of the reagent, the relevant operations of the experimenter, and the experimental environment. Our company only assumes responsibility for the reagent kit itself and does not bear any responsibility for sample consumption caused by using the reagent kit. Please fully consider the possible usage amount of the sample before use and reserve sufficient samples.