- Catalog No.
K137
- Size
1mL/10mL
- Storage
The kit should be stored at -20℃ away from light for one year.
- CAS No.
28718-90-3
- Staining Localization
Nucleus
- Applications
4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) Staining Solution is a staining solution suitable for nuclear staining of all common cells and tissues. It can be used directly for nuclear staining of fixed cells or tissues. DAPI is a cell-membrane-permeable blue fluorescent dye that, upon binding to double-stranded DNA, exhibits fluorescence more than 20 times stronger than that of DAPI alone. Compared to EB (ethidium bromide), it offers significantly higher sensitivity for staining double-stranded DNA.
DAPI staining is commonly used in apoptosis detection, with observation under a fluorescence microscope or detection by flow cytometry after staining. In addition, DAPI is also widely used for routine nuclear staining and for double-stranded DNA staining under certain specific conditions.
The maximum excitation wavelength of DAPI is 340 nm, and its maximum emission wavelength is 488 nm. Upon binding to double-stranded DNA, the maximum excitation wavelength shifts to 364 nm and the maximum emission wavelength shifts to 454 nm.
This DAPI solution is prepared in water at a concentration of 1 mg/mL. For nuclear staining, the recommended working concentration of DAPI is 0.5–10 μg/mL.
- Note
1.Perform detection on the same day after staining to prevent fluorescence quenching.
2.This product is irritant. Handle with care and avoid direct contact or inhalation.
3.Store protected from light to prevent quenching.
4.This product is intended for research use by professionals only. Not for clinical diagnosis or treatment, not for food or pharmaceutical use, and not to be stored in ordinary living quarters.
5.For your safety and health, please wear a lab coat and disposable gloves when handling.
