FineTest ELISA kit contributes to the research on cerebral ischemia reperfusion injury therapy. The immunoassay is designed to measure TNF-α and IL-1β concentration in supernatant.
Publication Details
Article Title: Ligustrazine Nanoparticle Hitchhiking on Neutrophils for Enhanced Therapy of Cerebral Ischemia-Reperfusion Injury
Journal Title: Advanced Science
DOI: 10.1002/advs.202301348
IF: 17.521
Abstract: Ischemic stroke is a refractory disease that endangers human health and safety owing to cerebral ischemia. Brain ischemia induces a series of inflammatory reactions. Neutrophils migrate from the circulatory system to the site of cerebral ischemia and accumulate in large numbers at the site of inflammation across the blood–brain barrier. Therefore, hitchhiking on neutrophils to deliver drugs to ischemic brain sites could be an optimal strategy. Since the surface of neutrophils has a formyl peptide receptor (FPR), this work modifies a nanoplatform surface by the peptide cinnamyl-F-(D)L-F-(D)L-F (CFLFLF), which can specifically bind to the FPR receptor. After intravenous injection, the fabricated nanoparticles effectively adhered to the surface of neutrophils in peripheral blood mediated by FPR, thereby hitchhiking with neutrophils to achieve higher accumulation at the inflammatory site of cerebral ischemia. In addition, the nanoparticle shell is composed of a polymer with reactive oxygen species (ROS)-responsive bond breaking and is encased in ligustrazine, a natural product with neuroprotective properties. In conclusion, the strategy of hitching the delivered drugs to neutrophils in this study could improve drug enrichment in the brain, thereby providing a general delivery platform for ischemic stroke or other inflammation-related diseases.
Keywords: Cerebral Ischemia-reperfusion Injury, Drug Delivery, Ligustrazine, Neutrophils, Reactive Oxygen Species (ROS)
Immunoassay
| FineTest Product | Sample | Detection Target |
| Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit(EM0183) | supernatant | TNF-α |
| Mouse IL-1β(Interleukin 1 Beta) ELISA Kit(EM0109) | IL-1β |
Validated Image
Figure Source: Adv Sci (Weinh). 2023 Apr 20;e2301348. doi: 10.1002/advs.202301348.
Figure 6. Treatment with T-TMP reduced the expression of ROS, apoptosis, and the production of inflammatory factors in the brain tissue of MCAO in mice.