Human ZP3(Zona pellucida sperm-binding protein 3) ELISA Kit

Manual
  • Specifications
Product name

Human ZP3(Zona pellucida sperm-binding protein 3) ELISA Kit

Catalogue No.

EH1363

Alias

ZP3(Zona pellucida sperm-binding protein 3) ELISA Kit, ZP3A ELISA Kit, ZP3B ELISA Kit, ZPC ELISA Kit, Zp-3 ELISA Kit, Zona pellucida protein C ELISA Kit, Zona pellucida glycoprotein 3 ELISA Kit, Sperm receptor ELISA Kit, ZP3A ELISA Kit, ZP3B ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

ZP3 ELISA Kit allows for the in vitro quantitative determination of ZP3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human ZP3(Zona pellucida sperm-binding protein 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ZP3 and the recovery rates were calculated by comparing the measured value to the expected amount of ZP3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-104 96
EDTA plasma(n=5) 85-104 98
heparin plasma(n=5) 86-93 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ZP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-105% 90-102% 91-102% 89-102%
EDTA plasma(n=5) 83-94% 87-101% 85-93% 85-99%
heparin plasma(n=5) 81-99% 85-95% 83-95% 85-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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