Human WISP3(WNT1-inducible-signaling pathway protein 3) ELISA Kit

Manual
  • Specifications
Product name

Human WISP3(WNT1-inducible-signaling pathway protein 3) ELISA Kit

Catalogue No.

EH0928

Alias

WISP3(WNT1-inducible-signaling pathway protein 3) ELISA Kit, CCN6 ELISA Kit, WISP-3 ELISA Kit, CCN family member 6 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

WISP3 ELISA Kit allows for the in vitro quantitative determination of WISP3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

93.75-6000pg/ml

Sensitivity

< 56.25pg/ml

Standard curve

Human WISP3(WNT1-inducible-signaling pathway protein 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of WISP3 and the recovery rates were calculated by comparing the measured value to the expected amount of WISP3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-104 97
EDTA plasma(n=5) 90-98 94
heparin plasma(n=5) 85-104 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WISP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-101% 88-102% 89-103% 92-100%
EDTA plasma(n=5) 82-95% 84-100% 83-100% 82-101%
heparin plasma(n=5) 80-90% 81-92% 81-96% 90-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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