Human VGF(Neurosecretory protein VGF) ELISA Kit

Manual
  • Specifications
Product name

Human VGF(Neurosecretory protein VGF) ELISA Kit

Catalogue No.

EH0769

Alias

VGF(Neurosecretory protein VGF) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

VGF ELISA Kit allows for the in vitro quantitative determination of VGF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human VGF(Neurosecretory protein VGF) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of VGF and the recovery rates were calculated by comparing the measured value to the expected amount of VGF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 95
EDTA plasma(n=5) 92-101 97
heparin plasma(n=5) 89-103 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 85-105% 89-102% 85-103%
EDTA plasma(n=5) 82-99% 87-101% 83-100% 82-94%
heparin plasma(n=5) 81-93% 81-97% 81-97% 84-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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