Human TOP2B(DNA topoisomerase 2-beta) ELISA Kit

Manual
  • Specifications
Product name

Human TOP2B(DNA topoisomerase 2-beta) ELISA Kit

Catalogue No.

EH1354

Alias

TOP2B ELISA Kit, DNA topoisomerase 2-beta ELISA Kit, DNA topoisomerase II, beta isozyme ELISA Kit, TOPIIB ELISA Kit, top2beta ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TOP2B ELISA Kit allows for the in vitro quantitative determination of TOP2B concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human TOP2B(DNA topoisomerase 2-beta) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TOP2B and the recovery rates were calculated by comparing the measured value to the expected amount of TOP2B in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 95-103 98
EDTA plasma(n=5) 92-98 95
heparin plasma(n=5) 86-104 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TOP2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-103% 85-105% 86-102% 86-104%
EDTA plasma(n=5) 91-99% 87-101% 89-100% 82-91%
heparin plasma(n=5) 80-99% 85-100% 85-100% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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