Human S1PR3(Sphingosine 1-phosphate receptor 3) ELISA Kit

Manual
  • Specifications
Product name

Human S1PR3(Sphingosine 1-phosphate receptor 3) ELISA Kit

Catalogue No.

EH0849

Alias

S1PR3 ELISA Kit, EDG3 ELISA Kit, LPB3 ELISA Kit, S1P3 ELISA Kit, EDG-3 ELISA Kit, Endothelial differentiation G-protein coupled receptor 3 ELISA Kit, S1P receptor 3 ELISA Kit, S1P receptor EDG3 ELISA Kit, S1P receptor Edg-3 ELISA Kit, sphingosine 1-phosphate receptor 3 ELISA Kit, Sphingosine 1-phosphate receptor Edg-3 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

S1PR3 ELISA Kit allows for the in vitro quantitative determination of S1PR3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human S1PR3(Sphingosine 1-phosphate receptor 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of S1PR3 and the recovery rates were calculated by comparing the measured value to the expected amount of S1PR3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-104 95
EDTA plasma(n=5) 96-101 98
heparin plasma(n=5) 95-103 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S1PR3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 88-104% 96-105% 89-100%
EDTA plasma(n=5) 82-101% 85-101% 83-100% 83-98%
heparin plasma(n=5) 87-98% 86-91% 84-97% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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