Human PDE4B(cAMP-specific 3′,5′-cyclic phosphodiesterase 4B) ELISA Kit

Manual
  • Specifications
Product name

Human PDE4B(cAMP-specific 3′,5′-cyclic phosphodiesterase 4B) ELISA Kit

Catalogue No.

EH0903

Alias

PDE4B ELISA Kit, DPDE4 ELISA Kit, PDE32 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PDE4B ELISA Kit allows for the in vitro quantitative determination of PDE4B concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human PDE4B(cAMP-specific 3′,5′-cyclic phosphodiesterase 4B) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDE4B and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4B in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 99
EDTA plasma(n=5) 88-102 96
heparin plasma(n=5) 86-100 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 89-104% 90-100% 85-96%
EDTA plasma(n=5) 83-99% 90-96% 90-101% 84-97%
heparin plasma(n=5) 80-98% 81-99% 80-95% 91-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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